Comparative Analysis of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres dna extraction kit

Relative Analysis of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Concentrating On Nucleic Acid Removal.

(LNJNbio Polystyrene Microspheres)

In the field of modern biotechnology, microsphere materials are commonly utilized in the removal and filtration of DNA and RNA due to their high certain area, great chemical stability and functionalized surface buildings. Among them, polystyrene (PS) microspheres and their obtained polystyrene carboxyl (CPS) microspheres are among the two most widely studied and applied materials. This post is provided with technical assistance and data analysis by Shanghai Lingjun Biotechnology Co., Ltd., intending to systematically contrast the efficiency differences of these 2 kinds of materials in the process of nucleic acid extraction, covering crucial indicators such as their physicochemical residential properties, surface alteration capacity, binding efficiency and healing price, and illustrate their applicable situations through experimental data.

Polystyrene microspheres are uniform polymer particles polymerized from styrene monomers with excellent thermal stability and mechanical stamina. Its surface area is a non-polar framework and typically does not have active practical teams. For that reason, when it is straight made use of for nucleic acid binding, it requires to depend on electrostatic adsorption or hydrophobic action for molecular fixation. Polystyrene carboxyl microspheres present carboxyl practical teams (– COOH) on the basis of PS microspheres, making their surface capable of additional chemical combining. These carboxyl teams can be covalently adhered to nucleic acid probes, proteins or other ligands with amino teams through activation systems such as EDC/NHS, thus attaining much more steady molecular addiction. Consequently, from a structural perspective, CPS microspheres have a lot more advantages in functionalization potential.

Nucleic acid removal typically includes actions such as cell lysis, nucleic acid launch, nucleic acid binding to strong phase carriers, washing to get rid of impurities and eluting target nucleic acids. In this system, microspheres play a core function as solid phase service providers. PS microspheres mainly rely on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding efficiency has to do with 60 ~ 70%, but the elution performance is reduced, just 40 ~ 50%. In contrast, CPS microspheres can not just make use of electrostatic results however also attain more solid fixation via covalent bonding, lowering the loss of nucleic acids throughout the washing process. Its binding performance can get to 85 ~ 95%, and the elution efficiency is likewise raised to 70 ~ 80%. Additionally, CPS microspheres are likewise dramatically much better than PS microspheres in terms of anti-interference capability and reusability.

In order to validate the performance differences in between the two microspheres in real procedure, Shanghai Lingjun Biotechnology Co., Ltd. conducted RNA removal experiments. The speculative samples were derived from HEK293 cells. After pretreatment with basic Tris-HCl buffer and proteinase K, 5 mg/mL PS and CPS microspheres were utilized for extraction. The results showed that the typical RNA return extracted by PS microspheres was 85 ng/ μL, the A260/A280 ratio was 1.82, and the RIN worth was 7.2, while the RNA return of CPS microspheres was enhanced to 132 ng/ μL, the A260/A280 ratio was close to the perfect value of 1.91, and the RIN value got to 8.1. Although the procedure time of CPS microspheres is somewhat longer (28 minutes vs. 25 minutes) and the expense is greater (28 yuan vs. 18 yuan/time), its extraction top quality is significantly improved, and it is preferable for high-sensitivity detection, such as qPCR and RNA-seq.

( SEM of LNJNbio Polystyrene Microspheres)

From the viewpoint of application circumstances, PS microspheres appropriate for large screening tasks and preliminary enrichment with reduced demands for binding specificity due to their low cost and simple operation. Nevertheless, their nucleic acid binding capacity is weak and quickly influenced by salt ion focus, making them inappropriate for long-term storage space or repeated usage. In contrast, CPS microspheres are suitable for trace example extraction as a result of their rich surface area useful groups, which promote further functionalization and can be made use of to build magnetic bead detection kits and automated nucleic acid removal systems. Although its preparation procedure is fairly intricate and the price is fairly high, it shows stronger flexibility in scientific research and clinical applications with rigorous demands on nucleic acid removal efficiency and purity.

With the rapid growth of molecular medical diagnosis, genetics editing, liquid biopsy and other fields, greater needs are put on the performance, purity and automation of nucleic acid extraction. Polystyrene carboxyl microspheres are gradually changing traditional PS microspheres because of their outstanding binding efficiency and functionalizable characteristics, coming to be the core option of a brand-new generation of nucleic acid extraction materials. Shanghai Lingjun Biotechnology Co., Ltd. is also constantly maximizing the fragment dimension circulation, surface thickness and functionalization efficiency of CPS microspheres and creating matching magnetic composite microsphere products to fulfill the needs of scientific diagnosis, scientific research study organizations and industrial customers for high-grade nucleic acid extraction services.

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